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說明: Tested species reactivityRabbitPublished species reactivityNot ApplicableHost / IsotypeGoat / IgGClassPolyclonalTypeSecondary AntibodyImmunogenGamma Immunoglobins Heavy and Light chainsConjugateAlexa Fluor? 488FormliquidConcentration2 mg/mlPurificationpurifiedStorage bufferPBS, pH 7.5Contains5mM sodium azideStorage Conditions4° C, store in darkTargetIgGCross AdsorptionAgainst human IgG, human serum, mouse IgG, mouse serum and bovine serumAntibody FormWhole Antibody
說明: Tested species reactivityGoatPublished species reactivityNot ApplicableHost / IsotypeDonkey / IgGClassPolyclonalTypeSecondary AntibodyImmunogenGamma Immunoglobins Heavy and Light chainsConjugateAlexa Fluor? 350FormLiquidConcentration2 mg/mlPurificationpurifiedStorage bufferPBS, pH 7.5Contains5mM sodium azideStorage Conditions4° C, store in darkTargetIgGCross AdsorptionAgainst rabbit, rat, mouse and human IgGAntibody FormWhole Antibody
說明: Supplierbio-radCode MAB360Host MouseConjugate UnconjugatedType PrimaryClonality MonoclonalClone GA5Protein P14136
說明: 在正常細胞中,磷脂酰絲氨酸 (PS)只分布在細胞膜脂質雙層的內側,而在細胞凋亡早期,細胞膜中的磷脂酰絲氨酸 (PS)由脂膜內側翻向外側。Annexin V是種分子量為35~36 kD 的Ca2+依賴性磷脂結合蛋白,與磷脂酰絲氨酸具有高度親和力,故可通過細胞外側暴露的磷脂酰絲氨酸與凋亡早期細胞的細胞膜結合。因此Annexin V被公認為檢測細胞早期凋亡的靈敏指標之。將Annexin V進行綠色熒光(FITC或EGFP)標記,以標記了的Annexin V作為探針,利用熒光顯微鏡或流式細胞儀可檢測細胞凋亡的發生。碘化丙啶(Propidium Iodide, PI)是種核酸染料,它不能透過正常細胞或早期凋亡細胞的完整的細胞膜,但對凋亡中晚期的細胞和壞死細胞,PI能夠透過細胞膜而使細胞核染紅。因此將Annexin V與PI匹配使用,就可以將處于不同凋亡時期的細胞區分來。